| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032033 |
| Chromosome: | chromosome 15 |
| Location: | 1207736 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g801718 | NCL58 | Nuclear Control of chloroplast-Like 58; (1 of 39) PF08373 - RAP domain (RAP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGCGCCCCAGCACGCCCTCCACCACCACCTCCATCCGCACCGAAAC |
| Internal bar code: | CAGTTTTTGAGTGGTCCTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3205 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGTCCAACATCCTGCTG |
| Suggested primer 2: | CTTTTCCGCTGATTGCTGCC |