Insertion junction: CLIP2.032050_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.032050
Chromosome:chromosome 10
Location:25737
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g417500 CYN20E,CYN20-5,CYN9 (1 of 1) PF00160//PF04969 - Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD (Pro_isomerase) // CS domain (CS); Cyclophilin 20-5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAAGTGGTAAGACAGGGCGGCGCGTCCCCCGGTTCTGGAACCTTCACG
Internal bar code:TGGATAATAAGGGGGGGTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3157
LEAP-Seq percent confirming:98.3607
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTCTCCACCACTAGCT
Suggested primer 2:GCAGTCTGTCTTGGGACCTC