Insertion junction: CLIP2.032070_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.032070
Chromosome:chromosome 17
Location:3270219
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g721900 COG5 Component of oligomeric golgi complex; (1 of 1) PF10392 - Golgi transport complex subunit 5 (COG5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCATATATCTCCCCCTCCTCCCCACAGGCTGATGCACGAGGAGCTCAAG
Internal bar code:TCCATACTGCTATGGTTAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3932
LEAP-Seq percent confirming:94.8718
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGAAGTCTCCAAGCAGGG
Suggested primer 2:GGGCAAGGGTGAGTGTGTAA