Insertion junction: CLIP2.032071_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.032071
Chromosome:chromosome 7
Location:5657000
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g352550 RDP3 Putative rhodanese domain phosphatase; (1 of 3) K18065 - Cdc25 family phosphatase [EC:3.1.3.48 1.20.4.-] (CDC25) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCACTTGCGCTGCCCACCACGCTGTCCGCACACACGCGCACCTGCTCC
Internal bar code:ATGGTGCCTAGCTATATCTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1197
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACGCTCATTGTGGACGT
Suggested primer 2:TTCAATGGTCGCTCGAACCA