| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032073 |
| Chromosome: | chromosome 9 |
| Location: | 5926227 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412050 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTATTTTGACCCGGACCGTAGCCGTGCTTGTGCCATCCCGGGCAAGG |
| Internal bar code: | AGTCTCGGTGTGCACGGCAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1904 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGAAGGTTTGGGCTTGC |
| Suggested primer 2: | GTGACCTGAACGAGTACGCA |