| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032093 |
| Chromosome: | chromosome 10 |
| Location: | 220609 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801097 | (1 of 48) IPR011050 - Pectin lyase fold/virulence factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGCGGGTCCGTCCCAACATTGCCTCTGCTGTTGCTGCTACTGCCACT |
| Internal bar code: | ATGAGGGTGCCGAAGATCCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2417 |
| LEAP-Seq percent confirming: | 89.1892 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATGCGTGTATGGTGAAC |
| Suggested primer 2: | CATGCGCTGTACATTGGACG |