| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032120 |
| Chromosome: | chromosome 13 |
| Location: | 2078032 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577050 | ELG7 | Exostosin-like glycosyltransferase 7; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAGGGTGACGGAGGGAGCCGAAGCAAACTACCGGCAACCAGGGTCAC |
| Internal bar code: | CTACACATCTATTTGCGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2579 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAACGCCACAGTACGAT |
| Suggested primer 2: | GCCTGCCTAGCATCATGGAT |