Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.032127 |
Chromosome: | chromosome 12 |
Location: | 5862664 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g533351 | CLPB5 | (1 of 3) K03695 - ATP-dependent Clp protease ATP-binding subunit ClpB (clpB); ClpB chaperone, Hsp100 family | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGCGGGCGTGGAGGTGGAGGGCGGCGCCAGCGAGGCCGAGAAGAAGG |
Internal bar code: | GGACTAAGTTCGACGGTAACGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 583 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 18 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAGGCTGACAGGTCTAGGG |
Suggested primer 2: | CGCTACGGTTTGCCATGATG |