Insertion junction: CLIP2.032127_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.032127
Chromosome:chromosome 12
Location:5862664
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533351 CLPB5 (1 of 3) K03695 - ATP-dependent Clp protease ATP-binding subunit ClpB (clpB); ClpB chaperone, Hsp100 family CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGGCGGGCGTGGAGGTGGAGGGCGGCGCCAGCGAGGCCGAGAAGAAGG
Internal bar code:GGACTAAGTTCGACGGTAACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:583
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGCTGACAGGTCTAGGG
Suggested primer 2:CGCTACGGTTTGCCATGATG