| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.032140 |
| Chromosome: | chromosome 1 |
| Location: | 3467052 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022550 | (1 of 15) 3.6.5.5 - Dynamin GTPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAGGGAGGGCTGGCTGCAAACAGGGCGGGCGCGCGGGGCAACAGGAG |
| Internal bar code: | CGCGTCTCGCATGGGTAAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 284 |
| LEAP-Seq percent confirming: | 4.54545 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTTCCACTCCCATTGTC |
| Suggested primer 2: | TTGCAGAAAACGCTCTTGGC |