Insertion junction: CLIP2.032144_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.032144
Chromosome:chromosome 13
Location:1539845
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g572850 FBB17 (1 of 6) PF00246 - Zinc carboxypeptidase (Peptidase_M14); Flagellar/basal body protein 17 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACCCGCGCCCACGCCCAGCCCACGCGCCCGCCCCCGCCCCCGCGCCGC
Internal bar code:TGTTATCCCGATTTACACATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:467
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCAACGAAATAGGTCCCA
Suggested primer 2:CCCAGTTTCGCTTGGTTTGG