| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.032145 |
| Chromosome: | chromosome 14 |
| Location: | 3333221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629550 | (1 of 1) K06963 - tRNA acetyltransferase TAN1 (TAN1, THUMPD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACTGAAGACTGCAGACGAAGTTGTTGGGGGGAGCGGAGTACAAGGC |
| Internal bar code: | GAGCCCGCTAAGTGGACGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 38.0952 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACACACACACCACACACA |
| Suggested primer 2: | TCTCGCCCCATCTCACCTAA |