Insertion junction: CLIP2.032161_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.032161
Chromosome:chromosome 2
Location:4354625
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g105287 (1 of 1) PF13821 - Domain of unknown function (DUF4187) (DUF4187) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTTCACCTGCGACAGGCGCACTGCTACTGCTACTTCTGCGGCACGGC
Internal bar code:GTCAAGTTGTACTCTGCAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2253
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TCGACACGGTAAAGCGACAA
Suggested primer 2:GGGAAGACACACACAGCCTT