| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.032165 |
| Chromosome: | chromosome 12 |
| Location: | 1092124 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491400 | (1 of 2) K02977 - small subunit ribosomal protein S27Ae (RP-S27Ae, RPS27A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCGACATCAGTTGGCTGCGATTGGAGCGGCTGCTGGCGAACACGAACG |
| Internal bar code: | ATAGAAGCAGGCTGATGGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2695 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCACTTACTCTTGGTCGT |
| Suggested primer 2: | GGTCCATGCAGTAAGCCCTT |