| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032166 |
| Chromosome: | chromosome 3 |
| Location: | 6638671 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195050 | (1 of 1) PTHR11732//PTHR11732:SF263 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCTCTTGCCCACCGGCTTGGCCAAAGCCCCGCTGTCCATCCCGGCC |
| Internal bar code: | CCGACCACTCCAAGGATTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4687 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACGATAGCGGGTCATAA |
| Suggested primer 2: | TTACCGCACATGCTGGGATT |