| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032193 |
| Chromosome: | chromosome 3 |
| Location: | 6270589 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191800 | (1 of 33) PF13855 - Leucine rich repeat (LRR_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGGCGGCGGGCGTGTTTGTATGGAGCGGCGGCGGCGCATACGCAGTC |
| Internal bar code: | ATTCATGTTAAAGGTAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4276 |
| LEAP-Seq percent confirming: | 89.7436 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAATCAAGAGTCCCACCG |
| Suggested primer 2: | GCGGGTGGTATGTAGCTGTT |