Insertion junction: CLIP2.032195_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.032195
Chromosome:chromosome 8
Location:1935677
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g367950 FAP323 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like; Cyclic nucleotide-monophosphate binding domain Flagellar Associated Protein 323 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGGGCCGGCGGCGGCGGTGCGGGGGGCTGAGGCCAGGAACACGGCCA
Internal bar code:CTTTTTTGCTGGAGACTACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2179
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:TGACGCGGTCTGAACATGAA
Suggested primer 2:GAGGAGTGTGTGGAGTGTGG