| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.032229 |
| Chromosome: | chromosome 10 |
| Location: | 2063290 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432650 | (1 of 1) 3.6.1.59 - 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase / M(7)GpppX pyrophosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGACCAGACCAGCTCGGACCAGCTGTACTGTCTGGCTCTGGTGGCGCG |
| Internal bar code: | ACGGATCTACGTGAGTCCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGTAGTAGGAGGGCTG |
| Suggested primer 2: | GGGTAAGCATCAGCCCAAGT |