| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.032258 |
| Chromosome: | chromosome 7 |
| Location: | 2498954 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329550 | VFL5 | Variable flagella protein 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGGCGGCGTGGAATCACTGGCGGCTGCGGTACTGCAGCGCAGCCGCA |
| Internal bar code: | CGTCAAGTTTCCGTCTTATACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3827 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGAATGAGGCGGAAATGC |
| Suggested primer 2: | CCATCCCCCATGAAACGGAA |