| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.032262 |
| Chromosome: | chromosome 8 |
| Location: | 3181422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376650 | CSR5 | Carbohydrate sulfotransferase-related 5; (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCGAGACAGCCAGGACGCAGCAGCGAGTCAGACAGGCGGTGCGGCGG |
| Internal bar code: | GAGAAGAAAGCAGGCCTAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2440 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTACCTTCCATCGGAGA |
| Suggested primer 2: | TCACACAGTCTGAACTCGCC |