| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032284 |
| Chromosome: | chromosome 6 |
| Location: | 3254583 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800653 | (1 of 1) 3.1.3.53 - [Myosin-light-chain] phosphatase / Myosin phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGGCGGAGGCAGCAGGGGGCGGCTGGCTGCGTATGTAAAGCGCACAG |
| Internal bar code: | CGGGACGACATGAGGATTGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2721 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTAAGCAGCATGAACGC |
| Suggested primer 2: | AGCAGCAGCAACAGATGGAT |