| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.032308 |
| Chromosome: | chromosome 1 |
| Location: | 6715489 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048000 | TPM1 | Tropomyosin 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACCGTATTCCCTGTGGACAGCGTCACAGCGCTCGCGCAGCCCTGGGCA |
| Internal bar code: | TATGCCTAATGTTCTTGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1726 |
| LEAP-Seq percent confirming: | 35.8491 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCATATTCACCGTGTGCG |
| Suggested primer 2: | GTCAGCCAGAAGCCCTGAAT |