Insertion junction: CLIP2.032319_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.032319
Chromosome:chromosome 12
Location:1868364
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g512200 MFT26 Major facilitator superfamily transporter; (1 of 1) PTHR23504//PTHR23504:SF15 - FAMILY NOT NAMED // PROTEIN ZINC INDUCED FACILITATOR 1-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCAGTCTCGCAGGTCTGTGTGGTTAGGGCAGCCAGTTGGGGTATGGG
Internal bar code:CTTATATAGGATCTGACCGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:788
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTCGTGTGTTGGGCTAT
Suggested primer 2:TTCATGGTCCGCGACTTTGA