Insertion junction: CLIP2.032340_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.032340
Chromosome:chromosome 17
Location:4506945
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g802092 (1 of 1) PF00050//PF05548//PF07648 - Kazal-type serine protease inhibitor domain (Kazal_1) // Gametolysin peptidase M11 (Peptidase_M11) // Kazal-type serine protease inhibitor domain (Kazal_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGGTCACACGAAAGCAATTTTAATGATGTGATGCGACGCATGTTGC
Internal bar code:TTTTTAATACTACTGTTAGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2252
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCTCGATTGGGAGAGTAC
Suggested primer 2:CATGTGGCGGATGAGCAATG