| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032342 |
| Chromosome: | chromosome 2 |
| Location: | 5050516 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111050 | AMT7,AMT1G | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAACGGCGGTTCCCCCCGCCCCCATGAACACACAGGGACATACACGCA |
| Internal bar code: | CCGTCAGCTTTATTCTACATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3928 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGGAACATCCTCCTCAA |
| Suggested primer 2: | AGCACTATTCCCGATACGCG |