| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032350 |
| Chromosome: | chromosome 10 |
| Location: | 5310076 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456600 | (1 of 13) PTHR13018//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // PHOSPHATE METABOLISM PROTEIN 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGGGAGTGGCGAATAAGACCTGACGCAGGCCAACTCATGGTGCACG |
| Internal bar code: | GGCTGTCTGTCCAACGGCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5893 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACACAACAGGCCAATCG |
| Suggested primer 2: | TGAAGAACCTGGGCGTCATC |