Insertion junction: CLIP2.032368_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.032368
Chromosome:chromosome 8
Location:1634493
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g365825 (1 of 1) 1.14.99.42 - Zeaxanthin 7,8-dioxygenase / Zeaxanthin 7,8(7',8')-cleavage dioxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGATAGTCGAGCTCCAGCGGCCACAGCTTAGACAGCTGGCCATGCTTA
Internal bar code:GTACTTGCATAGTATTACCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1251
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCCAAATCCGCACTGTG
Suggested primer 2:GGAATCGCGGGGGTTTATCT