Insertion junction: CLIP2.032379_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.032379
Chromosome:chromosome 12
Location:4236321
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g518107 (1 of 1) PF04182 - B-block binding subunit of TFIIIC (B-block_TFIIIC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACATTCACCCCACCCCTCCGCCTGCTGTACCAATGTGCCTGGCTACAG
Internal bar code:ATGAGCAGGTCGGGCTGTGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:230
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCACACACACATCTCCCACG
Suggested primer 2:TTCCACAGACGCTCAAGACC