Insertion junction: CLIP2.032390_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.032390
Chromosome:chromosome 3
Location:7660513
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g204100 ELG5 Exostosin-like glycosyltransferase 5; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAACTGCACTGCCCTATCTTCGCTCCAAACAATTCCTGAGTCCCTACCT
Internal bar code:AGGGGACTGTCTGCTGTTATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2799
LEAP-Seq percent confirming:94.6565
LEAP-Seq n confirming:124
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:131

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCTGCCCTTAACCAGTC
Suggested primer 2:CAGATGGCCAGGTCCTCATC