| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.032394 |
| Chromosome: | chromosome 1 |
| Location: | 224069 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001400 | ZMP1 | (1 of 1) 3.4.24.36 - Leishmanolysin / Promastigote surface endopeptidase; Zinc-metallopeptidase-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGGCAGCCTGGCCATTGAGCGCCGCCGCCTGCAGCAGGTGAGACCC |
| Internal bar code: | TATTGCATAATGTGTCGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGGGGTAGTAGGACAGG |
| Suggested primer 2: | ACATACCTCTTTGCCTGCCC |