| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032413 |
| Chromosome: | chromosome 12 |
| Location: | 9875171 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543902 | (1 of 1) 2.7.1.16 - Ribulokinase / L-ribulokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAACGCCGTGCCGAGGCACCGACATGCCCTGTCCCCGCCACGCCACG |
| Internal bar code: | TTAATTAGCAGTTACTTGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2656 |
| LEAP-Seq percent confirming: | 89.1089 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGCGAACCCCGGAATC |
| Suggested primer 2: | CGTGCTTGAGTGTGAGTCCT |