Insertion junction: CLIP2.032430_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.032430
Chromosome:chromosome 6
Location:1399907
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g259650 MPA7 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR10161 - TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCCTGCTCGTGCCAGTCACTGCACTAAAAGCTACTCCACCACCCTTG
Internal bar code:GCGCACCCGACCCCCACATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:465
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCACATCCTGTACCGCTTT
Suggested primer 2:TCCGGATAGGACAAGGCTCA