Insertion junction: CLIP2.032441_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.032441
Chromosome:chromosome 2
Location:4404846
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g105750 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCCGCACCTGCCCGGCGCGATCAACGACACCTTATGCGTCCCGTTCTC
Internal bar code:AAATGTCTGGTCGACGGACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2652
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:85

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCTTTTCGCAGGCCTGTT
Suggested primer 2:CGAGTCAAAAACAGTGCGCA