Insertion junction: CLIP2.032519_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.032519
Chromosome:chromosome 11
Location:3299709
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g476500 PAO7 Pheophorbide a oxygenase-related protein; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCAACTGCCGCAGGTGGTTGCGGCGGTTGCTGTTCAGCGGCACCAAC
Internal bar code:ATCGGTGGTACGTCGGGTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2128
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGCCATTCGCATTGTCAG
Suggested primer 2:CAGAGCTGCAGGACTGTCTC