Insertion junction: CLIP2.032540_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.032540
Chromosome:chromosome 1
Location:3874910
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025450 DRC9,FAP122 Nexin-dynein regulatory complex 9; (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATCACCTGACGCACAACATCCTTCCTGTCTGCGCTTGCCCCGCAGCT
Internal bar code:ACGATTTACGAATTTGGCCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1614
LEAP-Seq percent confirming:97.5
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTTGACACTGTCCCATCA
Suggested primer 2:GTCCAACTGCCTGAACGAGA