| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.032542 |
| Chromosome: | chromosome 3 |
| Location: | 3458327 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166950 | PGM5 | (1 of 1) K01834 - 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (PGAM, gpmA); Phosphoglycerate mutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTCCGGCATTGACACTGATTCGGACTCCCCACAGTCTGACCCCAGGC |
| Internal bar code: | GCAAAACGTGGGGGGGCGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1561 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCGAGGTGAACACGAA |
| Suggested primer 2: | AGCTGTTGCAGTGAGACTCC |