Insertion junction: CLIP2.032579_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.032579
Chromosome:chromosome 16
Location:2787754
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g662350 TSPS1,TPS2 (1 of 3) 2.4.1.15 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / UDP-glucose--glucose-phosphate glucosyltransferase; Trehalose-6-phosphate synthase, class I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACGCCCTGTTTGGGGGGCTGCTACAGCCGGACCTGCTACAGCCTCCC
Internal bar code:AAGATTATTTCGGGAAGAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:458
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGCTGCTGTTGTTGTT
Suggested primer 2:GGACGTGGTTTGCTGTAGGA