| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.032589 |
| Chromosome: | chromosome 1 |
| Location: | 3309074 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021300 | (1 of 2) IPR016140 - Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGACCTGCTGGAGCAGGTAGGAGCCGGCACTCCTGCTGGGCGCGTGTG |
| Internal bar code: | AGAAAATATATGGTTTATAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2542 |
| LEAP-Seq percent confirming: | 64.2105 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTGTTTTATCGGCATGC |
| Suggested primer 2: | GCAGCAAGACAACCTGAACG |