| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032602 |
| Chromosome: | chromosome 5 |
| Location: | 1500113 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234655 | (1 of 1) K17550 - protein phosphatase 1 regulatory subunit 7 (PPP1R7, SDS22) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAGGAACCGAGGCACAACGACGGGACGGGGTGTGAGGACTGATGGGGA |
| Internal bar code: | TGGTTGGCTTGGTAAGTATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACGGCATCTCCACTCTG |
| Suggested primer 2: | CGGCATACCCAAGGAAACCT |