Insertion junction: CLIP2.032610_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.032610
Chromosome:chromosome 7
Location:2016859
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325761 (1 of 2) IPR000104//IPR003439//IPR003593//IPR011527//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC transporter type 1, transmembrane domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTCCACGTTCCTCCGCCTGACCCTCTCGCTCGCTCCCTCCCTCTGCCT
Internal bar code:AGGTTATTTAAATCTCTTTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:397
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCACAAAACACCCACGAC
Suggested primer 2:CCCACACACACACACACAAC