Insertion junction: CLIP2.032610_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.032610
Chromosome:chromosome 7
Location:2016860
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325761 (1 of 2) IPR000104//IPR003439//IPR003593//IPR011527//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC transporter type 1, transmembrane domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGATAGCGAGTTTAGGTTGGTTGGCGATTATGTTGGGATGGCGCCGT
Internal bar code:AGGTTATTTAAATCTCTTTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:299
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACACACACACACACAAC
Suggested primer 2:GCTCACAAAACACCCACGAC