| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032614 |
| Chromosome: | chromosome 7 |
| Location: | 1779746 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800785 | (1 of 5) PTHR30405 - TRANSPOSASE | gene_edge/mRNA_edge/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGCCGGTGTTGTCTGGTTAGCGACCTGTGCTGACGAGGATGGCGTAG |
| Internal bar code: | GCGATACCTCAAACTGAAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4653 |
| LEAP-Seq percent confirming: | 97.4359 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCGCTATCATCGTGACA |
| Suggested primer 2: | CCACTCACCTTCACCCACTC |