Insertion junction: CLIP2.032626_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.032626
Chromosome:chromosome 6
Location:5903106
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g289800 CAM12 Calmodulin-like protein; (1 of 1) 2.3.1.23//2.3.1.51//2.3.1.67 - 1-acylglycerophosphocholine O-acyltransferase / Lysolecithin acyltransferase // 1-acylglycerol-3-phosphate O-acyltransferase // 1-alkylglycerophosphocholine O-acetyltransferase / Platelet-activating factor-synthesizing enzyme intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAAGGATGACGGCAGCGCTGGGTAGCGTGAGTACGAGCCACCTGAGG
Internal bar code:AATAGTCCTACGTCTCGATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1820
LEAP-Seq percent confirming:97.619
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGAGTGGTGGAAAGTGTA
Suggested primer 2:GCATCCAGGAACTCGAACCA