| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.032656 |
| Chromosome: | chromosome 12 |
| Location: | 5132095 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATTGACAAACACCGCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG |
| Internal bar code: | TTCAGTATGCTCGTAAAGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4495 |
| LEAP-Seq percent confirming: | 1.08696 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 182 |
| LEAP-Seq n unique pos: | 184 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCTAGCAGACCGTCACG |
| Suggested primer 2: | TAACTGCTGTGGTCCGTTCC |