| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032674 |
| Chromosome: | chromosome 5 |
| Location: | 629077 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800522 | (1 of 88) IPR011333 - POZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTTGAAGGTGTTGGTTCGAACGGCGCGGCGCCTGTCTTCCTCGCTAC |
| Internal bar code: | CGAGTGCCCAAGATTATAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGTGGGTTTGTGTGTGT |
| Suggested primer 2: | TACGGTGAAGAGGAGGGAGG |