| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.032682 |
| Chromosome: | chromosome 10 |
| Location: | 5618963 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458600 | FAP245 | Flagellar Associated Protein 245 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACCCCGAGCTGCAGGTGGGTGTGTGGTAGGGGGCAGCGCAGGGCGCG |
| Internal bar code: | ATTACAGATCGTGGGCCTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3048 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCTGCCTCCACCTCAAC |
| Suggested primer 2: | TACACGGCTGTTTCAGGGAC |