| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032773 |
| Chromosome: | chromosome 1 |
| Location: | 2599180 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015400 | (1 of 2) PF05542 - Protein of unknown function (DUF760) (DUF760) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGGATGTTGGGGGCTGTCGCATGCCAAGCCCTAGCAACCATGCCAT |
| Internal bar code: | GGACGTTTAAGGGAATTTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4043 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCGAGGTCTACTGATG |
| Suggested primer 2: | TGTAGCAGTGCTGTGTGAGG |