| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032801 |
| Chromosome: | chromosome 10 |
| Location: | 4487255 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGCCCGTAGCGCCGGTGCCCAGCAGGGTGCCGTCGAAGCGGAACTGC |
| Internal bar code: | GGGCCAGGCGTATTATTGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1709 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTGACAAGTGCCCAGCC |
| Suggested primer 2: | CGCATGGGAGATAGACGACC |