Insertion junction: CLIP2.032815_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.032815
Chromosome:chromosome 11
Location:1519860
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g468700 CPLD65,HCAR1 7-hydroxymethyl chlorophyll a reductase; (1 of 1) 1.17.7.2 - 7-hydroxymethyl chlorophyll a reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTCACCTGCGCCTCGAGCACCCAGCGACACCCTTTGCCGAGCTACCGT
Internal bar code:TTCGTCGAATCGAAGGTGACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:961
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CCGAGCTCGATACCAGCATT
Suggested primer 2:CTCCTCGCTCGTTTGCATTG