| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.032829 |
| Chromosome: | chromosome 14 |
| Location: | 1056675 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614300 | (1 of 1) 1.1.1.205 - IMP dehydrogenase / Inosinic acid dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCCATGGCGTTAGGGCTGGCGCCGAAACTTCCTAGCCGCCAGCACCA |
| Internal bar code: | GGGTTTTTAGTTGGAATGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3669 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGAGCTGACGTTGGACC |
| Suggested primer 2: | CAGCTTGGAGACAGTGTCGT |