| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.032832 |
| Chromosome: | chromosome 16 |
| Location: | 1770784 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654992 | (1 of 1) 2.1.1.225 - tRNA:m(4)X modification enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCGCGCCGCCCTGGGGACAGCAAGAGCGGCAAGGGAAAAGAAGACC |
| Internal bar code: | AACTATTTCTATCTGTAAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3445 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGAGAAGCCACGTGTAAG |
| Suggested primer 2: | TAGTCTTTGAGGTCCACGCG |